To investigate the effects of Baihe Gujin decoction drugserum and Si Ni decoction drugserum on expression of Tolllike receptor 2 and Tolllike receptor 4 on macrophage ,and the relationship between the ratio of TLR2 and TLR4 and the efficacy of antiinfective traditional Chinese medicine.RAW264.7 cells were incubated with the drugserum prepared with Baihe Gujin decoction and Si Ni decoction for 24 h.The mRNA and protein TLR2 and TLR4 expressed in macrophages respectively were then measured by RTPCR and FACS.The phagocytic and killing activity of the RAW264.7 cells interfered with the drugserum for 24 h were then detected after H37Ra stimulation.Meanwhile,we place a blank rabbit serum as a control group (Control).Compared with the control group and the Si Ni decoction group,the ratio of TLR2 and TLR4 within Baihe Gujin decoction group were reduced significantly both at mRNA and protein levels (P < 0.05) .Furthermore,48 h after intervention by Baihe Gujin decoction drugserum,the DNA content of Mycobacterium tuberculosis in macrophages was distinctly reduced (P<0.05).Taken together,these findings suggest that both Baihe Gujin decoction and Si Ni decoction drugserum can adjust the ratio of TLR2 and TLR4.It may be a theoretical basis for the treatment of diseases.