建立一种检测念珠菌甘露聚糖(mannan, Mn)的磁微粒化学发光法(magnetic particle chemiluminescence immunoassay, MPCLIA)：采用念珠菌Mn作为抗原免疫BALB/c小鼠制备单克隆抗体；使用全自动化学发光分析仪，应用双抗体夹心法，在反应体系中形成链霉亲和素-磁微粒-生物素标记抗体-念珠菌Mn-吖啶磺酰胺标记抗体复合物，建立检测念珠菌Mn的MPCLIA。 结果表明，空白限(limit of blank, LoB)为2.0 pg/mL, 检出限(limit of detection, LoD)为5.0 pg/mL；线性范围10.0～1 000.0 pg/mL；可重复性和精密度实验变异系数均小于10%；回收率85%～115%。 在检测样本中添加血红蛋白、胆红素或甘油三酯浓度分别为7 mg/mL、300 mg/L和7.5 mmol/L时，对检测结果没有显著影响。该方法与临床诊断结果有较好一致性（κ值为0.79）。 该研究建立的念珠菌Mn MPCLIA已达到体外诊断试剂的质量标准，积累足够样本数据后，有望成为念珠菌病的免疫学快速检测方法。 

A magnetic particle chemiluminescence immunoassay (MPCLIA) for the detection of Candida mannan (Mn) was established. Monoclonal antibody was prepared by immunizing BALB/c mice with Candida Mn as antigen. The MPCLIA for Candida Mn detection was established by using automatic chemiluminescence analyzer and double-antibody sandwich method. The complex of streptavidin-magnetic particle-biotin labeled-Candida Mn-acridine sulfonamide labeled antibody was formed in the reaction system. The results showed that the limit of blank (LoB) was 2.0 pg/mL and the limit of detection (LoD) was 5.0 pg/mL. The linear range was 10.0~1 000.0 pg/mL. The variation coefficients of repeatability and of precision  were both less than 10%. The recovery rate was 85%～115%. When the concentrations of hemoglobin, bilirubin or triacylglycerol (TAG) reached 7 mg/mL, 300 mg/L and 7.5 mmol/L, respectively, there was no significant interference on the laboratory results. The results generated with this method were in line with clinical diagnosis outcomes (κ=0.79). Altogether, the Candida Mn MPCLIA established in this study has met the detection standards for in vitro diagnostic reagents, and  is expected to become a rapid immunological detection method of Candidiasis after  sufficient sample data promise.