研究巨噬细胞极化对人前列腺癌PC-3细胞促血管形成的作用，并探讨其机制。本实验成功诱导正常男性外周血单核细胞为M0、M1、M2和TAM，发现它们在形态学上差异明显。ELISA检测各亚型巨噬细胞条件培养液(condition medium，CM)表明：TGF-β 在 TAM中显著高于其他亚型，IL-10高表达于M2 及 TAM，而IL-12在M1中高表达(P < 0.05)。RT-PCR结果表明： IL-6、IL-23与 CXCL9 在 M1中表达显著增高(P < 0.05)，以及miRNA let-7b 、let-7c比TAM表达显著增高(P < 0.01)。体外血管形成实验结果显示M2、TAM能增强PC-3细胞的促血管形成(P < 0.01)。因此，TAM亚型巨噬细胞促进PC-3细胞的血管形成机制可能与miRNA let-7b、let-7c高表达以及上述细胞因子的差异性表达有关。

This research aimed to investigate the effect of four subtypes of macrophage resulting from different polarization on angiogenesis of human prostate cancer PC-3 cells, and to address the underlying mechanism. Normal male peripheral blood monocytes were induced to differentiate into M0, M1, M2 and TAM subtypes which were significantly different in morphology. The expression of TGF-β was significantly increased in the CM of TAM, and IL-10 expressed highly in M2 and TAM, whereas IL-12 was significantly increased in M1(P < 0.05) as evidenced by ELISA. At the same time, the expressions of IL-6, IL-23 and CXCL9 at mRNA level in M1 were significantly increased as compared with M0, M2 and TAM as detected by RT-PCR(P < 0.05).let-7b and let-7c in TAM were significantly up-regulated compared with the other three subtypes(P < 0.01); M2 and TAM could promote the angiogenesis of PC-3 cells(P < 0.01). Therefore, the mechanism by which macrophage of TAM subtype to promote angiogenesis of PC-3 cells may be related to the expressions of miRNA let-7b and let-7c through regulating the expression of the cytokines examined in this study.