探讨Elmo1在中性粒细胞胞外诱捕网(neutrophil extracellular trap， NET)形成过程中的作用机制。分别提取WT小鼠和Elmo1敲除小鼠的中性粒细胞后，用PMA诱导NET形成。运用激光扫描共聚焦显微镜对NET的形成进行观察，用多功能酶标仪读取荧光强度值并进行定量分析。为了进一步探索Elmo1调节NET形成的分子机制，我们用Western blotting检测了NET形成过程中相关信号通路的激活情况，包括p38和Akt。结果发现，Elmo1敲除的条件下，NET的形成明显减少。同时，在Elmo1敲除小鼠的中性粒细胞中，PMA诱发的p38和Akt的激活也显著降低。抑制p38、Akt后，WT和Elmo1敲除型小鼠之间NET形成的差异消失。说明Elmo1通过p38和Akt参与上调NET的形成。上述结果提示Elmo1参与上调NET形成。

We aimed to investigate howElmo1 signaling regulates NET formation in neutrophils. Bone marrow neutrophils were isolated from wild type and Elmo1 knockout mice, and stimulated with PMA to induce NET formation, which was quantitatively analyzed by measuring the fluorescence intensity with laser scanning confocal microscope and Spectra Max i3. In order to further explore the molecular mechanism by which Elmo1 regulates NET formation, we detected the activation of intracellular signaling pathways such as p38 and Akt in NET formation by Western blotting. We found that NET formation was significantly reduced in Elmo1 knockout neutrophils. Meanwhile, Elmo1 deficiency resulted in reduced activation of p38 and Akt. With inhibition of p38 and Akt, the difference in NET formation between wild type and Elmo1 knockout neutrophils got abolished, implicating that Elmo1 regulates NET formation through p38 and Akt. Taken together, our data suggest that Elmo1 up-regulates NET formation in neutrophils.