To investigate the protective effect of camel milk on acute colitis induced by trinitro-benzene-sulfonic acid (TNBS) in mice and the underlying mechanism, 24 male BALB/c mice 6-8 weeks old were used to construct the acute colitis model by TNBS enema administration, and were randomly divided into two groups. The experimental groups were gavaged with camel milk, and the control group were gavaged with double distilled water. FACS was used to detect the percentages of immune cells and ELISA to detect the cytokine levels. The results showed that compared with the control group, the experimental group had the slower rate of body weight loss, the histological score decreased, the percentage of CD11b+Gr1+ cells increased, the percentage of CD8+T cells and IL-17+CD4+T cells decreased, the level of IL-17 in the supernatant of cell culture decreased, and the level of IL-10 increased. In conclusion, camel milk has a protective effect on TNBS-induced acute colitis in mice, which may be related to immune regulation.