骆驼奶对TNBS诱导的小鼠急性肠炎的保护作用

崔畅婉,于淼,吴思,等

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现代免疫学 ›› 2021, Vol. 41 ›› Issue (4) : 320-324.
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骆驼奶对TNBS诱导的小鼠急性肠炎的保护作用

  • 崔畅婉,于淼,吴思,王爽,孙峥嵘
作者信息 +

Protection effect of camel milk on TNBS induced mice acute colitis

  • CUI Chang-wan, YU Miao, WU Si, WANG Shuang, SUN Zheng-rong
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摘要

为探讨骆驼奶对2,4,6-三硝基苯磺酸(trinitro-benze-sulfonic acid,TNBS)诱导的小鼠急性肠炎的保护作用及相关机制,将24只6~8周龄的雄性BALB/c小鼠随机分为两组,实验组使用骆驼奶灌胃,对照组使用双蒸水灌胃,TNBS灌肠构建急性结肠炎模型。FACS检测免疫细胞百分比,ELISA测定细胞因子含量。结果显示,实验组小鼠比对照组体质量下降缓慢,组织学评分低,CD11b+Gr1+细胞百分比升高,CD8+ T细胞百分比下降,IL-17+ CD4+T细胞百分比降低,细胞培养上清液中IL-17水平降低,IL-10水平升高。由此,骆驼奶对TNBS诱导的小鼠急性肠炎具有保护作用,可能与免疫调节有关。

Abstract

To investigate the protective effect of camel milk on acute colitis induced by trinitro-benzene-sulfonic acid (TNBS) in mice and the underlying mechanism, 24 male BALB/c mice 6-8 weeks old were used to construct the acute colitis model by TNBS enema administration, and were randomly divided into two groups. The experimental groups were gavaged with camel milk, and the control group were gavaged with double distilled water. FACS was used to detect the percentages of immune cells and ELISA to detect the cytokine levels. The results showed that compared with the control group, the experimental group had the slower rate of body weight loss, the histological score decreased, the percentage of CD11b+Gr1+ cells increased, the percentage of CD8+T cells and IL-17+CD4+T cells decreased, the level of IL-17 in the supernatant of cell culture decreased, and the level of IL-10 increased. In conclusion, camel milk has a protective effect on TNBS-induced acute colitis in mice, which may be related to immune regulation.

关键词

骆驼奶 / 肠炎 / 2 / 4 / 6-三硝基苯磺酸

Key words

camel milk / colitis / trinitro-benzene-sulfonic acid

引用本文

导出引用
崔畅婉,于淼,吴思,等. 骆驼奶对TNBS诱导的小鼠急性肠炎的保护作用. 现代免疫学. 2021, 41(4): 320-324
CUI Chang-wan, YU Miao, WU Si, et al. Protection effect of camel milk on TNBS induced mice acute colitis. Current Immunology. 2021, 41(4): 320-324

基金

国家自然科学基金(81171581);辽宁省高等学校基本科研项目(LFWK201710);2018年辽宁省重点研发计划指导计划项目(2018225009)
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