为建立基于磁性微球可溶性fms样酪氨酸激酶1（soluble fms-like tyrosine kinase 1，sFlt-1）的时间分辨荧光免疫分析法（sFlt-1 time-resolved fluoroimmunoassay，sFlt-1-TRFIA），用磁珠偶联抗sFlt-1单克隆抗体，Eu³⁺(铕)标记抗sFlt-1单克隆抗体，采用双抗体夹心法建立基于磁珠的sFlt-1-TRFIA。结果显示，自制试剂的检测灵敏度为10.78 pg/mL，线性范围10.78～25 000 pg/mL；样品的回收率为98.23%～107.71%，分析内变异系数（intraassay coefficient of variation，CV）为5.29%～6.50%，分析间变异系数(intreassay coefficient of variation, CV)为6.55%～8.23%；与电化学发光法的检测结果比较，相关系数r2值为0.971 9。由此，基于磁性微球建立的sFlt-1-TRFIA灵敏度、特异度以及准确度等均符合临床应用要求，有望用于临床血清样本中sFlt-1浓度的检测。

This study aimed to establish a time-resolved fluoroimmunoassay based on magnetic microspheres soluble fms-like tyrosine kinase 1 (sFlt-1), the monoclonal antibody against sFlt-1 was labeled with magnetic beads and the monoclonal antibody against sFlt-1 was labeled with Eu³⁺. sFlt-1 time-resolved fluoroimmunoassay (sFlt-1-TRFIA) based on magnetic beads was established by the double-antibody sandwich method. The results showed that the sensitivity of the method was 10.78 pg/mL, and the linear range was 10.78～25 000 pg/mL. The average recovery rate of the sample was 98.23%～107.71%, the intraassay coefficient of variation (CV) was 5.29%～6.50%, and the intreassay CV was 6.55%～8.23%. The expression level of serum sFlt-1 was compared with the results of chemiluminescent immunoassay, and the correlation coefficient (r2-value) was 0.971 9. In conclusion, the sensitivity, specificity and accuracy of sFlt-1-TRFIA based on magnetic microspheres all meet the requirements of clinical application, and this method can be used to detect the concentration of sFlt-1 in patient serum sample.