为探讨长链非编码RNA（long non-coding RNA，lncRNA）排斥性引导分子骨形态发生蛋白辅助受体b反义RNA 1 （repulsive guide molecule bone morphogenetic protein coreceptor b antisense RNA 1, RGMB-AS1）对类风湿关节炎（rheumatoid arthritis，RA）滑膜成纤维细胞增殖、迁移和侵袭的影响及分子机制，选取20例活动期和缓解期RA患者滑膜组织及20例正常滑膜组织，用qRT-PCR检测lncRNA RGMB-AS1和miR-129-5p的表达水平；分离培养RA滑膜成纤维细胞，将其分为si-NC组、si-RGMB-AS1组、pcDNA组、pcDNA-RGMB-AS1组、miR-NC组、miR-129-5p组、si-RGMB-AS1+anti-miR-NC组、si-RGMB-AS1+anti-miR-129-5p组；用MTT试验检测细胞活性；Transwell试验检测细胞迁移和侵袭； Western blotting检测细胞周期蛋白D1（Cyclin D1）、细胞周期蛋白依赖性激酶抑制剂1A（p21）、基质金属蛋白酶（matrix metalloproteinase，MMP）-2、MMP-9、MMP-3、MMP-13蛋白表达；荧光素酶报告基因试验检测lncRNA RGMB-AS1和miR-129-5p的靶向关系。结果显示，与正常滑膜组织比较，缓解期和活动期RA患者滑膜组织中lncRNA  RGMB-AS1表达水平升高，miR-129-5p表达水平降低（P<0.05）；与缓解期RA患者滑膜组织比较，活动期RA患者滑膜组织中lncRNA RGMB-AS1表达水平升高，miR-129-5p表达水平降低（P<0.05）。干扰lncRNA RGMB-AS1表达或过表达miR-129-5p后，RA滑膜成纤维细胞活性降低，迁移、侵袭细胞数减少，Cyclin D1、MMP-2、MMP-9、MMP-3、MMP-13表达水平降低，p21表达水平升高（P<0.05）。lncRNA RGMB-AS1靶向调控miR-129-5p，抑制miR-129-5p表达逆转了干扰lncRNA RGMB-AS1表达对RA滑膜成纤维细胞增殖、迁移和侵袭的作用。该研究提示，干扰lncRNA RGMB-AS1表达可能通过上调miR-129-5p抑制RA滑膜成纤维细胞增殖、迁移和侵袭。

This study aimed to explore the effect of long non-coding RNA（lncRNA） repulsive guide molecule bone morphogenetic protein coreceptor b antisense RNA 1 (RGMB-AS1) on the proliferation, migration, and invasion of rheumatoid arthritis （RA）synovial fibroblasts and the underlying molecular mechanism. Twenty synovial tissues from  patients with active or remission RA and 20 normal synovial tissues were collected. qRT-PCR was conducted to detect the expressions of lncRNA RGMB-AS1 and miR-129-5p. RA synovial fibroblasts were isolated, cultured, and divided into the si-NC group, si-RGMB-AS1 group, pcDNA group, pcDNA-RGMB-AS1 group, miR-NC group, miR-129-5p group, si-RGMB-AS1+anti-miR-NC group, and si-RGMB-AS1+anti-miR-129-5p group. MTT assay was used to evaluate cell proliferation. Transwell assay was used to detect cell migration and invasion. Western blotting was used to detect protein expressions of Cyclin D1, cyclin-dependent kinase inhibitor 1A (p21), matrix metalloproteinase (MMP)-2, MMP-9, MMP-3, and MMP-13. The luciferase report experiment was performed to detect the interaction between lncRNA RGMB-AS1 and miR-129-5p. The results showed that compared to normal synovial tissues, the expression of lncRNA RGMB-AS1 was increased in the synovial tissues of patients with remission and active RA, whereas the expression of miR-129-5p was decreased (P<0.05). Compared to the synovial tissues of patients with RA in remission, the expression of lncRNA RGMB-AS1 in the synovial tissues of patients with active RA was increased while the expression of miR-129-5p decreased (P<0.05). Notably, interfering with the expression of lncRNA RGMB-AS1 by miRNA or overexpression of miR-129-5p both caused decreased cell proliferation activity as well as reduced migrating and invasive cells and decreased expressions of Cyclin D1, MMP-2, MMP-9, MMP-3, and MMP-13. In contrast, the expression of p21 was increased (P<0.05). LncRNA RGMB-AS1 targeted miR-129-5p, and inhibiting miR-129-5p expression reversed the effects of interfering lncRNA RGMB-AS1 expression on proliferation, migration and invasion of RA synovial fibroblasts. This study suggests that lncRNA RGMB-AS1 regulates the proliferation, migration, and invasion of RA synovial fibroblasts by targeting miR-129-5p.